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姚斌
发布日期:2009/6/22  阅读:9274次

 

男, 博士, 研究员, 博士生导师,营养与饲料研究室。

1.学习、工作简历

1984.9-1988.7, 华中农业大学, 微生物学专业, 学士 1988.9-1991.7, 中国农业大学, 作为病理学专业, 硕士 1991.9-1994.7, 中国农业科学院生物技术研究所, 分子生物学专业, 博士 1994,7-至今, 中国农业科学院饲料研究所, 从事饲料生物技术工作。

2.研究方向和成果

在国内率先开展生物活性饲料添加剂的分子生物学与基因工程的研究,建立了国内饲料研究中第一个分子生物学实验室,研究方向主要集中在饲料用酶制剂及其它生物活性物质的分子生物学和基因工程改良、高效表达目标产品的重组微生物生物反应器的构建等,目前研究进一步扩展到食品、环保、医药等多个领域,取得多项国际领先的研究成果。

已发表研究论文130余篇(其中SCI文章60), 申请发明专利30项。指导硕士生25, 博士生12名。在酶制剂的产业化方面, 5个产品实现产业化生产, 5年实现11项次的技术转让, 并实现了向发达国家—美国的技术转让(专利许可)

任第二届国家农业转基因生物安全委员会委员, 中国微生物学会酶工程专业委员会、北京生物工程学会农业生物技术委员会、饲料添加剂和添加剂预混合饲料生产许可证专家审核委员会、中国石油与化学工业协会科技图书编审委员会委员, 《生物工程学报》、《微生物学报》编委。

1999年被评为中国农业科学院跨世纪学科带头人,2001年获国家“863十五周年先进个人、重要贡献奖。主持完成的研究成果“利用基因工程酵母生产植酸酶”获得2001年度国家科学技术进步二等奖。2004年获农业部有突出贡献的中青年专家称号, 同年, 入选“新世纪百千万人才工程国家级人选”。2005年入选中国农业科学院一级杰出人才、同年获得政府特殊津贴。

 

3.团队成员

杨培龙
男, 博士, 副研究员, 生于1976.7, 现工作于中国农业科学院饲料研究所。
主要从事饲料微生物工程研究,内容包括各种环境饲用微生物资源筛选和利用、饲料用酶编码基因的高通量筛选分离和饲料用酶的高效表达研究。主持国家自然资源平台项目、国家“863”和国家转基因重大专项课题,总计经费200多万元。参与国家级课题研究10余项。目前已发表国内核心期刊论文13篇,SCI论文28篇,其中作为第一作者或通讯作者的分别为5和4篇。申请中国发明专利12项,授权1项,申请PCT专利一项。

孟昆, 男, 博士, 副研究员, 生于1971.9, 现工作于中国农业科学院饲料研究所。
研究方向为微生物分子生物学与基因工程。现主持一项国家863计划重点项目子课题—工业酶的分子改造和工程化技术(耐热植酸酶的研制)。建立了从特殊环境中快速、高通量筛选及定向改造植酸酶基因的技术体系,获得包括热稳定性在内的综合性质优良的新植酸酶。同时作为主要参加人参与国家973课题、948课题、中央级科研院所社会公益研究专项、国家基础平台项目及科研院所技术开发研究专项课题等共8项,参与申请国家发明专利或国际PCT专利7项。相关工作对丰富微生物酶资源,提高工程酶在不同领域的应用以保持其在国内外研究的领先水平起到积极促进的作用。在国内外研究刊物上发表论文共22篇,其中SCI论文13篇。

相关文章:

2008年
1. Huoqing Huang, Peilong Yang, Huiying Luo, Huigui Tang, Na Shao, Tiezheng Yuan, Yaru Wang, Yingguo Bai, Bin Yao*. High-level expression of a truncated 1,3-1,4-β-D-glucanase from Fibrobacter succinogenes in Pichia pastoris by optimization of codons and fermentation. Applied Microbiology and Biotechnology, 2008, 78(1): 95-103
2. P.J. Shi, K. Meng, Z.G. Zhou, Y.R. Wang, Q.Y. Diao and B. Yao*. The Host Species Affects the Microbial Community in the Goat Rumen. Letters Applied Microbiology, 2008, 46(1): 132-135
3. Tiezheng Yuan, Peilong Yang, Yaru Wang, Kun Meng, Huiying Luo, Wei Zhang, Ningfeng Wu, Yunliu Fan, Bin Yao*. Heterologous expression of a gene encoding a thermostable -galactosidase from Alicyclobacillus acidocaldarius. Biotech Letters, 2008, 30(2): 343-348
4. Xiaoxue Wang, Ningfeng Wu,*, Jun Guo, Xiaoyu Chu, Jian Tian , Bin Yao, Yunliu Fan. Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Biochemical and Biophysical Research Communications, 2008, 365(3): 453-458
5. Fu J, Huang H, Meng K, Yuan T, Yao B, Shi Y, Ouyang P*. A Novel Cold-Adapted Phospholipase A1 from Serratia sp. xjF1: Gene Cloning, Expression and Characterization. Enzyme and Microbial Technology, 2008, 42 (1) 187–194
6. Li YN, Yang PL, Meng K, Wang YR, Luo HY, Wu NF, Fan YL, Yao B*. Gene Cloning, Expression and Characterization of a Novel beta-Mannanase from Bacillus circulans CGMCC 1416. Journal of Microbiology and Biotechnology, 2008, 18(1): 160-166
7. Yuhong Zhang, Kun Meng, Yaru Wang, Huiying Luo, Peilong Yang, Pengjun Shi, Ningfeng Wu, Yunliu Fan, Jiang Li, Bin Yao*. A novel proteolysis-resistant lipase from keratinolytic Streptomyces fradiae var. k11. Enzyme and Microbial Technology, 2008, 42(4): 346-352
8. Feng Xu; Kun Meng, Yaru Wang, Huiying Luo, Peilong Yang , Ningfeng Wu, Yunliu Fan, Bin Yao*. Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Protein Expression and Purification, 2008, 58(2): 175-183
9. Pengjun Shi, Huoqing Huang, Yaru Wang, Huiying Luo, Bo Wu, Kun Meng, Peilong Yang, Bin Yao*. A novel phytase gene appA from Buttiauxella sp. GC21 isolated from grass carp intestine. Aquaculture, 2008, 275(1-4): 70-75
10. Z. ZHOU*, Z. REN, H. ZENG, and B. YAO. Apparent digestibility of various feedstuffs for bluntnose black bream Megalobrama amblycephala Yih. AQUACULUTRE NUTRITION, 2008, 14(2):153-165
11. Dawei Fu§, Huoqing Huang§, Huiying Luo, Yaru Wang, Peilong Yang, Kun Meng, Yingguo Bai, Ningfeng Wu, Bin Yao*. A highly pH-stable phytase from Yersinia kristeensenii: cloning, expression, and characterization. Enzyme and Microbial Technology, 2008, 42(6): 499-505
12. Ning Li, Peilong Yang, Yaru Wang, Huiying Luo, Kun Meng, Ningfeng Wu, Yunliu Fan, Bin Yao*. Cloning, expression, and characterization of a protease-resistant xylanase from Streptomyces fradiae var. k11. Journal of Microbiology and Biotechnology, 2008, 18(3): 410-416
13. Yuchun Liu$, Zhigang Zhou$, Bin Yao, Penguin Shi, Suxu He, Linn Benjaminsen Hølvold, Einar Ringø*. The effect of intraperitoneal injection of immunostimulatory substances on allochthonous gut microbiota of Atlantic salmon (Salmo salar L.) determined using Denaturing Gradient Gel Electrophoresis (DGGE). Aquaculture Research, 2008, 39(6): 635-646
14. Jian Tian, Xuexia Guo, Xiaoyu Chu, Ningfeng Wu*, Jun Guo, Bin Yao. Predicting the protein family of Methyl Parathion Hydrolase. Int J Bioinformatics Research and Applications, 2008, 42(2): 201-210
15. Rumei Chen, Guangxing Xue, Ping Chen, Bin Yao, Wenzhu Yang, Qianli Ma, Yunliu Fan*, Zuoyu Zhao, Mitchell C. Tarczynski, Jinrui Shi. Transgenic maize plants expressing a fungal phytase gene. Transgene Res, 2008, 17(4): 633-643
16. Hui-Ying Luo, Li-Hong Miao, Cheng-Xiang Fang, Pei-Long Yang, Ya-Ru Wang, Peng-Jun Sh, Bin Yao*, Yun-Liu Fan. Nesterenkonia flava sp. nov., isolated from paper mill effluent. International Journal of Systematic and Evolutionary Microbiology, 2008, 58(8): 1927-1930
17. Na Shao, Huoqing Huang Kun Meng Huiying Luo Yaru Wang Peilong Yang Bin Yao*. Cloning, Expression and Characterization of a New Phytase from the Phytopathogenic Bacterium, Pectobacterium wasabiae DSMZ 18074. Journal of Microbiology and Biotechnology, 2008, 18(7): 1221-1226
18. Ning Li, Kun Meng, Yaru Wang, Pengjun Shi, Huiying Luo, Yingguo Bai, Peilong Yang, Bin Yao*. Cloning, expression and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9. Applied Microbiology and Biotechnology, 2008, 80(2): 231-240
19. Huoqing Huang, Huiying Luo, Yaru Wang, Dawei Fu, Na Shao, Guozeng Wang, Peilong Yang, Bin Yao*. A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under the low pH and strong pepsin conditions. Applied Microbiology and Biotechnology, 2008, 80(3): 417-426
20. Yanan CAO, Huoqing HUANG, Kun MENG, Peilong YANG, Pengjun SHI, Yaru WANG, Huiying LUO, Zhifang ZHANG, Ningfeng WU, Bin YAO*. Cloning and Functional Expression of A α-Galactosidase from Yersinia pestis biovar Microtus str. 91001. Bioscience Biotechnology and Biochemistry, 2008, 72(8): 2203-2205

2009年:
21. Zhigang Zhou$, Yuchun Liu$, Pengjun Shi, Suxu He, Bin Yao*, E. Ringø. Molecular characterization of the autochthonous microbiota in the gastrointestinal tract of adult yellow grouper (Epinephelus awoara) cultured in cages. Aquaculture, 2009, 286(3-4): 184-189
22. Huiying Luo, Yaru Wang, Hui Wang, Jun Yang, Yuhui Yang, Huoqing Huang, Peilong Yang, Yingguo Bai, Pengjun Shi, Yunliu Fan, Bin Yao*. A novel highly acidic β-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris. Applied Microbiology and Biotechnology, 2009, 82(3): 453-461
23. Huoqing Huang$, Pengjun Shi$, Yaru Wang, Huiying Luo, Na Shao, Guozeng Wang, Peilong Yang and Bin Yao*. Gene diversity of beta-propeller phytase in the intestinal contents of grass carp insight into the major phosphorus release from phytate in nature. Applied and Environmental Microbiology, 2009, 75(6): 1508-1516
24. Hui-Ying Luo, Ya-Ru Wang, Li-Hong Miao, Pei-Long Yang, Peng-Jun Shi, Cheng-Xiang Fang, Bin Yao,* Yun-Liu Fan. Nesterenkonia alba sp. nov., an alkaliphilic actinobacterium isolated from the black liquor treatment system of a cotton pulp mill. International Journal of Systematic and Evolutionary Microbiology, 2009, 59(4): 863 – 868
25. Zhigang Zhou $,*, Yuchun Liu$, Suxu He, Pengjun Shi, Xiuhua Gao, Bin Yao*, Einar Ringø. Effects of dietary potassium diformate (KDF) on growth performance, feed conversion and intestinal bacterial community of hybrid tilapia (Oreochromis niloticus♀×O. aureus♂). Aquaculture, 2009, 291(1-2): 89-94
26. Ning Li, Pengjun Shi, Peilong Yang, Yaru Wang, Huiying Luo, Yingguo Bai, Zhigang Zhou, Bin Yao*. A xylanase with high pH stability from Streptomyces sp. S27 and its carbohydrate-binding module with/without linker region truncated versions. Applied Microbiology and Biotechnology, 2009, 83(1): 99-107
27. Huoqing Huang, Na Shao, Yaru Wang, Huiying Luo, Peilong Yang, Zhigang Zhou,  Zhichun Zhan, Bin Yao. A novel beta-propeller phytase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. Applied Microbiology and Biotechnology, 2009, 83(2): 249-259
28. Weina Gu$, Huoqing Huang$, Kun Meng, Peilong Yang, Dawei Fu, Huiying Luo, Yaru Wang, Bin Yao*, Zhichun Zhan. Gene cloning, expression and characterization of a novel phytase from Dickeya paradisiacal. Applied Biochemistry and Biotechnology, 2009, 157(2): 113-123
29. ZHI-GANG ZHOU1, SUXU HE1, YUCHUN LIU, PENGJUN SHI, GUANGXIANG HUANG, AND BIN YAO. The effects of dietary yeast culture or short-chain fructo-oligosaccharides on the intestinal autochthonous bacterial communities in juvenile hybrid tilapia, Oreochromis niloticus♀×O. aureus♂. Journal of the World Aquaculture Society(accepted)
30. Peilong Yang, Yanan Li, Yaru Wang, Kun Meng, Huiying Luo, Tiezheng Yuan , Yingguo Bai, Zhichun Zhan, Bin Yao*. A Novel β-mannanase with High Specific Activity from Bacillus circulans CGMCC1554: Gene Cloning, Expression and Enzymatic Characterization. Applied Biochemistry and Biotechnology(accepted). DOI: 10.1007/s12010-008-8364-3
31. Ning Li, Pengjun Shi, Peilong Yang, Yaru Wang, Huiying Luo, Yingguo Bai, Zhigang Zhou, Bin Yao*. Cloning, expression, and characterization of a new Streptomyces sp. S27 xylanase for which xylobiose is the main hydrolysis product. Applied Biochemistry and Biotechnology(accepted). DOI: 10.1007/s12010-008-8411-0
32. Bo Wu$, Pengjun Shi$, Jiang Li, Yaru Wang, Kun Meng, Yingguo Bai, Huiying Luo, Peilong Yang, Zhigang Zhou, Bin Yao*. A new aminopeptidase from the keratin-degrading strain Streptomyces fradiae var. k11. Applied Biochemistry and Biotechnology(accepted)
33. Zhigang Zhou$, Pengjun Shi$, Suxu He, Yuchun Liu, Guangxiang Huang, Bin Yao*, Einar Ringø. Identification of adherent microbiota in the stomach and intestine of emperor red snapper (Lutjanus sebae Cuvier) using 16S rDNA-DGGE. Aquaculture Research(accepted)
34. Yanan Cao, Yaru Wang, Kun Meng, Yingguo Bai1, Pengjun Shi, Huiying Luo, Peilong Yang, Zhigang Zhou, Zhifang Zhang, Bin Yao*. A novel protease-resistant α-galactosidase with high hydrolytic activity from Gibberella sp. F75: gene cloning, expression and enzymatic characterization. Applied Microbiology and Biotechnology(accepted)
35. Huoqing Huang, Huiyibg Luo, Yaru Wang, Dawei Fu, Na Shao, Peilong Yang, Kun meng, Bin Yao*. A Novel Low-temperature-active Phytase from Erwinia carotovora var. carotovota ACCC 10276. Journal of Microbiology and Biotechnology(accepted)
36. Dawei Fu$, Huoqing Huang$, Kun Meng, Yaru Wang, Huiying Luo, Peilong Yang, Tiezheng Yuan, Bin Yao*. Improvement of Yersinia frederiksenii Phytase Performance by a Single Amino Acid Substitution. Biotechnology and Bioengineering(accepted)
37. Zhang Yuhong, Pengjun Shi, Wanli Liu, Kun Meng, Yingguo Bai, Guozeng Wang, Zhichun Zhan, Bin Yao*. Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture. Journal of Microbiology and Biotechnology(accepted)
38. Junpei Zhou, Huoqing Huang, Kun Meng, Pengjun Shi, Yaru Wang, Huiying Luo, Peilong Yang, Yingguo Bai, Bin Yao*. Cloning of a new xylanase gene from Streptomyces sp. TN119 using a modified thermal asymmetric interlaced-PCR specific for GC-rich genes and biochemical characterization. Applied Biochemistry and Biotechnology(accepted)
39. Yanan, Cao, Yaru Wang, Huiying Luo, Pengjun Shi, Kun Meng, Zhigang Zhou, Zhifang Zhang, Bin Yao*. Molecular Cloning and Expression of a Novel Protease-resistant GH-36 α-Galactosidase from Rhizopus sp. F78 ACCC 30795. Journal of Microbiology and Biotechnology(accepted)
40. Xiaodan Liu, Kun Meng, Yaru Wang, Pengjun Shi, Tiezheng Yuan, Peilong Yang, Huiying Luo, Yingguo Bai, Bin Yao*. Gene cloning, expression and characterization of an alpha-galactosidase from Pedobacter nyackensis MJ11 CGMCC 2503 with potential as an aquatic feed additive. World Journal of Microbiology and Biotechnology(accepted)
41. Yingguo Bai, Peilong Yang, Yaru Wang, Pengjun Shi, Huiying Luo, Kun Meng, Bo Wu and Bin Yao*. Phytase production by fermentation of recombinant Pichia pastoris in monosodium glutamate wastewater. World Journal of Microbiology and Biotechnology(accepted)
42. Shuxu He$, Zhigang Zhou$*, Yuchun Liu, Pengjun Shi, Bin Yao*, Einar Ringø , Ilkyu Yoon. Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA®) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus ♀×O. aureus ♂) cultured in cages. Aquaculture(accepted)
43. Huiying Luo, Yaru Wang, Jiang Li, Hui wang, Jun Yang, Yuhui Yang, Huoqing Huang, YunliuFan, Bin Yao*. Cloning, expression and characterization of a novel acidic xylanase, XYL11B, from the acidophilic fungus Bispora sp. MEY-1. Enzyme and Microbial Technology(accepted)
44. Hui Wang, Huiying Luo, Yingguo Bai, Yaru Wang, Peilong Yang, Pengjun Shi, Wei Zhang, Yunliu Fan, Bin Yao*. An Acidophilic β-Galactosidase from Bispora sp. MEY-1 with High Lactose Hydrolytic Activity under Simulated Gastric Conditions. Journal of Agricultural and Food Chemistry(accepted)
45. Kun Meng, Yaru Wang, Peilong Yang, Huiying Luo, Yingguo Bai, Pengjun Shi, Tiezheng Yuan, Rui Ma, Bin Yao*. Rapid detection and quantification of zearalenone-producing Fusarium species by targeting the zearalenone synthase gene PKS4. Food Control(accepted)

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